Protein Labeling Reagents
Biotium 5-TAMRA-PEO3-amine
5-TAMRA-PEO3-amine (Tetramethylrhodamine-PEO3-amine) has a long water-soluble PEG spacer separating the dye and the amine functional group. The dye can be coupled to activated carboxy groups or sulfonyl chlorides. Properties: Ex/Em (MeOH) = 544/571 nm; Dark purple solid soluble in DMSO, DMF, or H2O; Store at 4°C and protect from light; C 37 H 42 F 6 N 4 O 11; MW: 832.74
Biotium 5-(and-6-)-((N-(5-aminopentyl)amino)carbonyl) Tetramethylrhodamine (Tetramethylrhodamine cadaverine) (mixed isomer)
INA (5-iodonaphthyl-1-azide) is a lipophilic photoreactive probe, which has been used to selectively label membrane-embedded cysteine residues of proteins. Properties: Light yellow solid soluble in DMSO or DMF; Store at 4°C and protect from light; C30H34N4O4; MW: 295.08.
Biotium Mix-n-Stain™ CF488A Small Ligand Labeling Kit
Mix-n-Stain™ Small Ligand Labeling Kits are designed for rapid labeling of low molecular weight (MW 150 -5000) ligands without a purification step. Suitable ligands or substrates include SNAP-tag, CLIP-tag™ and HaloTag ligand derivatives that have an aliphatic amine. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF488A dye has excitation/emission at 490/515 nm. It is suitable for labeling ligands for cell surface staining.
Biotium ViaFluor 647 Live Cell Microtubule Stain, 50uL
ViaFluor Live Cell Microtubule Stains are cell-permeable taxol probes for imaging the microtubule cytoskeleton in live cells. Available with blue, green, or far-red fluorescence.
Medchemexpress LLC Sulfo-NHS-LC-biotin sodium | 191671-46-2 | MFCD00078534 | 98.0% | 556.59 | C20H29N4NaO9S2 | 10 MG
Sulfo-NHS-LC-Biotin sodium is a water-soluble, amine-reactive sulfo-NHS ester of biotin with a long-chain spacer (LC) that enables efficient labeling of primary amines on proteins and other biomolecules. It is formulated as the sodium salt to improve aqueous solubility and is suited for selective cell-surface biotinylation, detection, and affinity capture applications.
- Amine-reactive sulfo-NHS ester for efficient biotinylation.
- Long-chain spacer reduces steric hindrance and preserves biomolecule function.
- Water-soluble sodium salt suitable for aqueous labeling of surface proteins.
- Limited membrane permeability for selective cell-surface labeling.
- High purity and well-characterized for reproducible results.
- Soluble in DMSO (approx. 175 mg/mL); store sealed and away from moisture.
Revvity Health Sciences Inc IVISense Vascular 680 Fluorescent Probe (AngioSense)
IVISense Vascular 680 Fluorescent Probe (AngioSense)
Biotium CF594 Hydrazide
Our CF dye hydrazides are bright, extremely water-soluble and nontoxic. These properties make the dyes excellent fixable polar tracers for microinjection into cells. Hydrazides also can be used for labeling aldehyde or ketone groups, however, for this application we recommend using the more reactive CF dye aminooxy forms. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Deep-red fluorescent CF594 dye has excitation/emission at 593/614 nm.
Biotium CF568 Tyramide
CF dye tyramide conjugates can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Red fluorescent CF568 dye has excitation/emission at 562/583 nm.Also see Biotium's CF Dye Tyramide Amplification Kits.
Biotium CF405L Succinimidyl Ester
CF Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. 405 nm-excitable green fluorescent CF405L dye has excitation/emission at 395/545 nm.
Biotium FAM SE, 5 and 6 isomers
5(6)-FAM SE (full name: 5-(and-6)-Carboxyfluorescein, succinimidyl ester mixed isomers) is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5. The amide linkage from the coupling reaction is much more stable than the thiourea linkage formed from the coupling of an amine and an isothiocyanate. Properties: Ex / Em = 495/519 nm; Yellow solid soluble in DMSO or DMF; Store at -20°C and protect from light; C 25 H 15 NO 9; MW: 473.4; [117548-22-8]
Biotium Tyramide Amplification Kit with HRP Streptavidin and Biotin-XX Tyramide
Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to the conventional avidin-biotinylated complex procedure. The kit contains HRP streptavidin Biotin-XX tyramide. The kit contains sufficient reagent for 50-150 slides.