Protein Labeling Reagents
Biotium Nuclear Membrane(NM97), CF488A conjugate, 0.1mg/mL
This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the nuclear membrane expressed in human cells. It can be used to stain the nuclear membrane in cell or tissue preparations and can be used as a marker of the nuclear membrane in subcellular fractions. It produces a ring pattern around the nucleus of cells of normal and malignant cells and may be used to stain the nuclear membrane of cells in fixed or frozen tissue sections. ,The nuclear envelope (also known as the perinuclear envelope, nuclear membrane, nucleolemma or karyotheca) is the double membrane of the nucleus that encloses genetic material in eukaryotic cells. It separates the contents of the nucleus (DNA in particular) from the cytosol (cytoplasm). Numerous nuclear pores are present
Biotium HIF1α(HIF1A-84), CF488A conjugate, 0.1mg/mL
HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stab
Biotium HIF1α(HIF1A-84), CF488A conjugate, 0.1mg/mL
HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stab
Biotium CD28(C28/77), CF488A conjugate, 0.1mg/mL
Recognizes a glycoprotein of 44-88 kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Biotium CD28(C28/77), CF488A conjugate, 0.1mg/mL
Recognizes a glycoprotein of 44-88 kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Sigma Aldrich Fine Chemicals Biosciences Cardiogreen polymethine dye | 3599-32-4 | MFCD00013078 | 100MG
Cardiogreen polymethine dye | Mol Wt: 774.96 | 3599-32-4 | MFCD00013078 | 100MG
Cambridge Isotope Laboratories 3-MeSO2-DDE (unlabeled) 40 ug/mL in nonane CP 95% 1 2 mL
3-MeSO2-DDE (unlabeled) 40 ug/mL in nonane CP 95% 1 2 mL
![eMolecules Broadpharm / BP Fluor 594 Cadaverine / 1mg / 599128700 / BP-25577 / / / [null] / 806.950 / C40H46N4O10S2](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502468095.JPG-250.jpg)
eMolecules Broadpharm / BP Fluor 594 Cadaverine / 1mg / 599128700 / BP-25577 / / / [null] / 806.950 / C40H46N4O10S2
Broadpharm / BP Fluor 594 Cadaverine / 1mg / 599128700 / BP-25577 / / / [null] / 806.950 / C40H46N4O10S2
Biotium CD5(CD5/54/F6), CF640R conjugate, 0.1mg/mL
Recognizes a 67 kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma ( ) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.Primary antibodies are available purified, or with a selection of fluorescent CF Dye
![eMolecules Broadpharm / Sulfo-Cy35 alkyne / 1mg / 761705697 / BP-28917 / / / [null] / 1028.310 / C41H40K3N3O13S4](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502462498.JPG-250.jpg)
eMolecules Broadpharm / Sulfo-Cy35 alkyne / 1mg / 761705697 / BP-28917 / / / [null] / 1028.310 / C41H40K3N3O13S4
Broadpharm / Sulfo-Cy35 alkyne / 1mg / 761705697 / BP-28917 / / / [null] / 1028.310 / C41H40K3N3O13S4
Biotium CellBrite™ Steady 488 Membrane Staining Kit | 100 labelings
CellBrite™ Steady Membrane Staining Kits allow fluorescence imaging of cell surface for up to several days in culture. The CellBrite™ Steady Dyes are unique fluorescent membrane probes that distribute between the cell surface and intracellular compartments, so cells retain cell surface staining over time. With the use of CellBrite™ Steady Enhancer, intracellular staining can be reduced or eliminated for imaging of cell outlines or boundaries.
Sartorius pHrodo Orange Cell Labeling Kit, Real-Time Phagocytosis Quantification, Compatible With IncuCyte System, 1 Kit
The Sartorius IncuCyte pHrodo Orange Cell Labeling Kit provides a reliable solution for quantifying phagocytosis and efferocytosis using live-cell imaging. It features a unique pH-sensitive fluorophore that offers low fluorescence at neutral pH and a significant increase in fluorescence under acidic conditions. This enables real-time evaluation of cellular behaviors affected by various factors, setting it apart from other assays.